From National Institute of Standards and Technology (US)
July 09, 2021
Alex Boss
alexandra.boss@nist.gov
(301) 975-3611
A coral disease called growth anomalies (GAs) is depicted here in the coral species Porites compressa, a reef-building species found off the coast of Hawaii. GAs can cause tumorlike protrusions that affect both the coral skeleton and its soft tissues. Credit: E. Andersson/NIST.
Two different field images depicting the coral species Porites compressa, a reef-building coral off of the coast of Hawaii. The corals are affected by growth anomalies (GAs), a disease that can cause tumorlike protrusions that affect both the coral skeleton and its soft tissue. The GAs are the larger protrusions on the corals. Credit: R. Day/NIST.
Coral reefs are a favorite spot for scuba divers and are among the world’s most diverse ecosystems. For example, the Hawaiian coral reefs, known as the “rainforests of the sea,” host over 7,000 species of marine animals, fishes, birds and plants. But coral reefs are facing serious threats, including a number of diseases that have been linked to human activity.
To understand the connection between human activity and a type of tumorlike disease called growth anomalies (GAs), researchers at the National Institute of Standards and Technology (NIST) have collaborated with the U.S Geological Survey (USGS) and the National Oceanic and Atmospheric Administration (NOAA) (US) to use an emerging molecular profiling method to identify 18 small molecules that promise to help them better understand the series of molecular reactions that lead to the disease.
GAs affect both the coral skeleton and its soft tissues. Scientists don’t know the cause of the disease or how it spreads but have hypothesized that there is a strong correlation between GA prevalence in coral colonies and human population density nearby.
Almost all types of corals are made of hundreds to millions of individual soft-bodied animals called polyps. The polyps secrete calcium carbonate to form a hard skeleton that lays the foundation for the coral colony. GAs affect corals through irregular and accelerated growth of their skeleton, causing it to be less dense and filled with holes. This results in a tumorlike mass in the skeleton of a coral colony with fewer polyps and a diminished ability to reproduce.
Shallow water corals receive food like carbohydrates and oxygen as a byproduct of photosynthesis from the symbiotic relationship they have with zooxanthellae, photosynthetic algae that live inside coral tissues. GAs can lead to fewer symbiotic zooxanthellae and therefore less energy being absorbed from photosynthesis.
Even though GAs do not typically directly lead to coral death, they do affect the overall health of coral colonies and can pose an ecological threat to coral populations. To analyze the disease, NIST researchers chose the coral species Porites compressa as their target sample.
This coral species is known as the “finger” or “hump” coral and is part of the stony coral family, which is “one of the important reef-building species in Hawaii,” said NIST chemist Tracey Schock. “They lay the foundation for the coral reef.”
P. compressa is found in shallow lagoons off the Hawaiian Islands, and the researchers obtained their coral samples from Kaneohe Bay, Oahu. The bay has been studied widely as a site affected by human activity such as sewage discharge and metal pollution. GAs have previously been observed in the coral species there.
In order to analyze and study GAs in P. compressa, researchers turned to the field of metabolomics, which is the study of small molecules, such as those making up living organisms found in tissues, blood or urine. These small molecules, known as metabolites, are the intermediate and end products in a linked series of biochemical reactions known as molecular pathways in an organism.
Some examples of such small molecules include sugars like glucose, amino acids, lipids and fatty acids. Their production can be influenced by genetic and environmental factors and can help researchers better understand the biochemical activity of tissue or cells. In this case, chemical analysis of metabolites provides significant information that helps researchers understand the physiology of the disease.
For their study, researchers sampled a coral colony that had both healthy and diseased tissue. They split up their samples so they could assess the healthy coral and diseased coral separately. They also had a separate adjacent sample that was free of diseased tissue.
The samples were frozen in liquid nitrogen, and then freeze-dried for practical sample processing while maintaining metabolic integrity. The researchers then separated the diseased parts from the healthy colony using a hammer and stainless-steel chisel and collected the tissue from the skeleton with a brush. In one of the final stages of the sample preparation, they chemically extracted the metabolites from the coral tissue using a combination of methanol, water and chloroform.
“The method is novel for coral studies,” said Schock. “With metabolomics, it is critical to preserve the state of all metabolites in a sample at the time of collection. This requires halting all biochemical activity using liquid nitrogen and maintaining this state until chemical extraction of the metabolome. The complexity of a coral structure necessitates stringent collection and processing protocols.”
The researchers then produced a metabolomic analysis of the coral samples by using a reproducible profiling technique known as proton nuclear magnetic resonance (1H NMR).
The 1H NMR technique exposes the coral extract to electromagnetic fields and measures the radio frequency signals released by the hydrogens in the sample. The various kinds of metabolites are revealed by their unique signals which inform of their chemical environment. NMR detects all signals from the magnetic nuclei within a sample, making it an unbiased “all-in-one” technique. Two-dimensional NMR experiments that can identify both hydrogens (1H) and their directly bound carbon (13C) atoms provide more chemical information, giving confidence in the accuracy of the identities of the various metabolites within a sample.
The study identified 18 different metabolites and a new GA morphological form in P. compressa. The researchers found that GA tumors have distinct metabolite profiles compared with healthy areas of the same coral colony and detected specific metabolites and metabolic pathways that may be important for these profile differences. They also discovered that the loss of internal pH regulation is seemingly responsible for the hollow skeletons that are a characteristic of GAs.
“We have not only characterized new aspects of GA physiology, but have also discovered candidate pathways that provide a clear path forward for future research efforts aiming to further understand GA formation and coral metabolism, in general,” said Schock.
As studies of this type accumulate, the researchers envision a database that could pull together coral metabolite information from multiple coral species into an accessible location for all scientists.
Collaborating with other researchers in different fields could increase understanding of the biological impacts of this disease on coral colonies. “We are going to learn which species are tolerant and which species are sensitive to stresses, and the physiological adaptations or mechanisms of both types will be important to conservation efforts,” said Schock.
For now, the researchers hope these findings will be helpful for other scientists analyzing coral species and ultimately be beneficial for the coral reefs themselves, potentially aiding efforts to better preserve them.
See the full article here.
five-ways-keep-your-child-safe-school-shootings
Please help promote STEM in your local schools.
National Institute of Standards and Technology (US)‘s Mission, Vision, Core Competencies, and Core Values
Mission
To promote U.S. innovation and industrial competitiveness by advancing measurement science, standards, and technology in ways that enhance economic security and improve our quality of life.
NIST’s vision
NIST will be the world’s leader in creating critical measurement solutions and promoting equitable standards. Our efforts stimulate innovation, foster industrial competitiveness, and improve the quality of life.
NIST’s core competencies
Measurement science
Rigorous traceability
Development and use of standards
NIST’s core values
NIST is an organization with strong values, reflected both in our history and our current work. NIST leadership and staff will uphold these values to ensure a high performing environment that is safe and respectful of all.
Perseverance: We take the long view, planning the future with scientific knowledge and imagination to ensure continued impact and relevance for our stakeholders.
Integrity: We are ethical, honest, independent, and provide an objective perspective.
Inclusivity: We work collaboratively to harness the diversity of people and ideas, both inside and outside of NIST, to attain the best solutions to multidisciplinary challenges.
Excellence: We apply rigor and critical thinking to achieve world-class results and continuous improvement in everything we do.
Background
The Articles of Confederation, ratified by the colonies in 1781, contained the clause, “The United States in Congress assembled shall also have the sole and exclusive right and power of regulating the alloy and value of coin struck by their own authority, or by that of the respective states—fixing the standards of weights and measures throughout the United States”. Article 1, section 8, of the Constitution of the United States (1789), transferred this power to Congress; “The Congress shall have power…To coin money, regulate the value thereof, and of foreign coin, and fix the standard of weights and measures”.
In January 1790, President George Washington, in his first annual message to Congress stated that, “Uniformity in the currency, weights, and measures of the United States is an object of great importance, and will, I am persuaded, be duly attended to”, and ordered Secretary of State Thomas Jefferson to prepare a plan for Establishing Uniformity in the Coinage, Weights, and Measures of the United States, afterwards referred to as the Jefferson report. On October 25, 1791, Washington appealed a third time to Congress, “A uniformity of the weights and measures of the country is among the important objects submitted to you by the Constitution and if it can be derived from a standard at once invariable and universal, must be no less honorable to the public council than conducive to the public convenience”, but it was not until 1838, that a uniform set of standards was worked out. In 1821, John Quincy Adams had declared “Weights and measures may be ranked among the necessities of life to every individual of human society”.
From 1830 until 1901, the role of overseeing weights and measures was carried out by the Office of Standard Weights and Measures, which was part of the U.S. Coast and Geodetic Survey in the Department of the Treasury.
Bureau of Standards
In 1901 in response to a bill proposed by Congressman James H. Southard (R- Ohio) the National Bureau of Standards was founded with the mandate to provide standard weights and measures and to serve as the national physical laboratory for the United States. (Southard had previously sponsored a bill for metric conversion of the United States.)
President Theodore Roosevelt appointed Samuel W. Stratton as the first director. The budget for the first year of operation was $40,000. The Bureau took custody of the copies of the kilogram and meter bars that were the standards for US measures, and set up a program to provide metrology services for United States scientific and commercial users. A laboratory site was constructed in Washington DC (US) and instruments were acquired from the national physical laboratories of Europe. In addition to weights and measures the Bureau developed instruments for electrical units and for measurement of light. In 1905 a meeting was called that would be the first National Conference on Weights and Measures.
Initially conceived as purely a metrology agency the Bureau of Standards was directed by Herbert Hoover to set up divisions to develop commercial standards for materials and products. Some of these standards were for products intended for government use; but product standards also affected private-sector consumption. Quality standards were developed for products including some types of clothing; automobile brake systems and headlamps; antifreeze; and electrical safety. During World War I, the Bureau worked on multiple problems related to war production even operating its own facility to produce optical glass when European supplies were cut off. Between the wars Harry Diamond of the Bureau developed a blind approach radio aircraft landing system. During World War II military research and development was carried out including development of radio propagation forecast methods; the proximity fuze and the standardized airframe used originally for Project Pigeon; and shortly afterwards the autonomously radar-guided Bat anti-ship guided bomb and the Kingfisher family of torpedo-carrying missiles.
In 1948, financed by the United States Air Force the Bureau began design and construction of SEAC: the Standards Eastern Automatic Computer. The computer went into operation in May 1950 using a combination of vacuum tubes and solid-state diode logic. About the same time the Standards Western Automatic Computer, was built at the Los Angeles office of the NBS by Harry Huskey and used for research there. A mobile version- DYSEAC- was built for the Signal Corps in 1954.
Due to a changing mission, the “National Bureau of Standards” became the “National Institute of Standards and Technology (US)” in 1988.
Following September 11, 2001, NIST conducted the official investigation into the collapse of the World Trade Center buildings.
Organization
NIST is headquartered in Gaithersburg, Maryland, and operates a facility in Boulder, Colorado, which was dedicated by President Eisenhower in 1954. NIST’s activities are organized into laboratory programs and extramural programs. Effective October 1, 2010, NIST was realigned by reducing the number of NIST laboratory units from ten to six. NIST Laboratories include:
Communications Technology Laboratory (CTL)
Engineering Laboratory (EL)
Information Technology Laboratory (ITL)
Center for Neutron Research (NCNR)
Material Measurement Laboratory (MML)
Physical Measurement Laboratory (PML)
Extramural programs include:
Hollings Manufacturing Extension Partnership (MEP), a nationwide network of centers to assist small and mid-sized manufacturers to create and retain jobs, improve efficiencies, and minimize waste through process improvements and to increase market penetration with innovation and growth strategies;
Technology Innovation Program (TIP), a grant program where NIST and industry partners cost share the early-stage development of innovative but high-risk technologies;
Baldrige Performance Excellence Program, which administers the Malcolm Baldrige National Quality Award, the nation’s highest award for performance and business excellence.
NIST’s Boulder laboratories are best known for NIST‑F1 which houses an atomic clock. NIST‑F1 serves as the source of the nation’s official time. From its measurement of the natural resonance frequency of cesium—which defines the second—NIST broadcasts time signals via longwave radio station WWVB near Fort Collins in Colorado, and shortwave radio stations WWV and WWVH, located near Fort Collins and Kekaha in Hawai’i, respectively.
NIST also operates a neutron science user facility: the NIST Center for Neutron Research (NCNR). The NCNR provides scientists access to a variety of neutron scattering instruments which they use in many research fields (materials science; fuel cells; biotechnology etc.).
The SURF III Synchrotron Ultraviolet Radiation Facility is a source of synchrotron radiation in continuous operation since 1961. SURF III now serves as the US national standard for source-based radiometry throughout the generalized optical spectrum. All NASA-borne extreme-ultraviolet observation instruments have been calibrated at SURF since the 1970s, and SURF is used for measurement and characterization of systems for extreme ultraviolet lithography.
The Center for Nanoscale Science and Technology (CNST) performs research in nanotechnology, both through internal research efforts and by running a user-accessible cleanroom nanomanufacturing facility. This “NanoFab” is equipped with tools for lithographic patterning and imaging (e.g., electron microscopes and atomic force microscopes).
Committees
NIST has seven standing committees:
Technical Guidelines Development Committee (TGDC)
Advisory Committee on Earthquake Hazards Reduction (ACEHR)
National Construction Safety Team Advisory Committee (NCST Advisory Committee)
Information Security and Privacy Advisory Board (ISPAB)
Visiting Committee on Advanced Technology (VCAT)
Board of Overseers for the Malcolm Baldrige National Quality Award (MBNQA Board of Overseers)
Manufacturing Extension Partnership National Advisory Board (MEPNAB)
Measurements and standards
As part of its mission, NIST supplies industry, academia, government, and other users with over 1,300 Standard Reference Materials (SRMs). These artifacts are certified as having specific characteristics or component content, used as calibration standards for measuring equipment and procedures, quality control benchmarks for industrial processes, and experimental control samples.
Handbook 44
NIST publishes the Handbook 44 each year after the annual meeting of the National Conference on Weights and Measures (NCWM). Each edition is developed through cooperation of the Committee on Specifications and Tolerances of the NCWM and the Weights and Measures Division (WMD) of the NIST. The purpose of the book is a partial fulfillment of the statutory responsibility for “cooperation with the states in securing uniformity of weights and measures laws and methods of inspection”.
NIST has been publishing various forms of what is now the Handbook 44 since 1918 and began publication under the current name in 1949. The 2010 edition conforms to the concept of the primary use of the SI (metric) measurements recommended by the Omnibus Foreign Trade and Competitiveness Act of 1988.
sciencesprings 