From Penn Today : “From corals to humans-a shared trigger for sperm to get in motion” 

From Penn Today

at

U Penn bloc

University of Pennsylvania

November 22, 2021
Katherine Unger Baillie

1
Hemaphroditic coral, like this Montipora capitata, release both eggs and sperm into the water. New findings from Penn biologists reveal that the mechanism by which sperm begin to move is both pH-dependent and similar to the pathways used in a variety of other creatures, including humans. Image: Courtesy of the Barott Laboratory.

If sperm can’t swim, life can’t go on. And a new study suggests that when evolution hit upon an effective strategy for making sperm move, it stuck with it.

A molecular pathway governing sperm motility is shared between corals, sea urchins, and even humans, according to research by a team from Penn’s School of Arts & Sciences. The mechanism is regulated by a pH sensor that signals when sperm are to begin swimming. The work, led by Kelsey Speer, a postdoctoral researcher in the lab of Katie Barott in the Department of Biology, appears in the journal PNAS.

Climate change, which is making the oceans more not only warmer but also more acidic, and localized disturbances, such as sedimentation, may threaten the process.

“When we started this project, nobody to our knowledge had looked at the mechanism that controlled coral sperm motility,” says Speer, the study’s first author. “We were really interested in what drives this process in the ocean, because that’s a part of their life cycle that is very vulnerable.”

“There’s so much diversity in sperm between species, so to find that this pathway was as conserved as it was, was surprising,” adds Barott, senior author on the paper. “I think this work highlights how important it is to regulate this function. Animals are dependent on these pathways functioning in order to make the next generation. If sperm don’t work, that’s the end.”

Sperm tend to be finicky and vulnerable, highly sensitive to their environment. Too warm? Males don’t produce sperm. Too acidic? Sperm don’t swim. Coral sperm have the odds stacked particularly tall against them. The hermaphroditic creatures only reproduce a few nights each year, timed with the new moon. They release both eggs and sperm into the open ocean, where sperm must swim through the water column, hoping for a fruitful match.

2
To capture sperm for their study, the Penn biologists conducted careful field work in Kaneohe Bay, Hawaii, where the coral Montipora capitata reproduce only a few nights each year. Image: Courtesy of Katie Barott.

In contrast to coral sperm, which have been little studied, sea urchins serve as a model organism for studying sperm. But despite their appearance, sea urchins are much more closely related to humans than to coral, and the signalling cascade responsible for setting their sperm in motion is also highly similar to that of vertebrates. Thus the Penn team was curious to see how regulation of coral sperm motility compared.

They started with a clue that corals may possess a similar mechanism.

“There is a really ancient pH-sensing enzyme that our lab had studied for a while that was present in corals,” Speer says. “It’s present in human sperm and it’s present in sea urchin sperm and we wondered, ‘Hey, it’s present in coral sperm too. What could it be controlling?’”

To find out, the researchers waited until one of those new-moon nights in Kaneohe Bay, Hawaii, to scoop up the egg-sperm bundles released by the coral Montipora capitata. Acting quickly, they took the sperm back to the lab, holding them in a sodium-free seawater. “What it does is it prevents all these signaling pathways from operating, so they’re frozen in an immobile state,” says Barott. “Then you can add a chemical to artificially raise their pH, and the sperm start swimming right away.”

3
The Penn team labeled the enzyme sAC in sperm with a green fluorescent marker, enabling them to track its activity in the lab. The genetic sequence encoding sAC in coral bore many similarities to the equivalent enzyme in sea urchins as well as vertebrates. Image: Courtesy of Kelsey Speer.

Upon this activation, the researchers were able to monitor the activity of the enzyme of interest, soluble adenylyl cyclase (sAC) and cyclic AMP, the messenger molecule it produces, while also tracking how well the sperm were moving. Their experiments confirmed that sAC activity was required for sperm to swim; when the enzyme was blocked, the sperm flagella—the “tails”—moved weakly.

Comparing the genetic sequence of the M. capitata sAC to the sAC from a sea urchin species, Speer, Barott, and colleagues noted significant similarities, with about 50% of the sequence being the same overall, and identical sequences at key sites for the enzyme’s catalytic activity.

“We looked at previously published datasets that catalog every mRNA that would become a protein in these cells, so we could get an idea of the molecular machinery in place to regulate sperm motility in these species,” says Barott.

Interestingly, M. capitata contained multiple different forms of sAC, some of which more closely resembled versions present in mammals. In follow-up work, the team hopes to explore how these different forms are operating in the corals, as well as in other model marine organisms.

Looking at other molecular players in the sperm activation pathways initiated by sAC, the researchers found several shared by sea urchins as well as both other coral species, members of the Cnidarian phylum.

“If you’re thinking about the difference in the last common ancestor between humans and Cnidarians—that was a heck of a long time ago,” Speer says. “The fact that the core of this mechanism has been conserved between these two species is really neat. I think it speaks to the fact that it’s a really good system, so nobody needed to replace it with something better.”

With a basic picture of coral sperm motility in place, Barott’s lab hopes to pursue additional experiments that get at the question of how changing environmental conditions could alter the organism’s reproductive success.

“Both us and colleagues who study this species of coral have seen huge differences in the amount of sperm become mobile from year to year, and it does look like climate change, especially heat stress, can play a big role in knocking down sperm motility,” Barott says. “Now that we have this toolkit, we can do these climate-change type of experiments and understand more about the dynamics of this pathway and how it changes in periods of stress.”

4
With coral reefs under threat from climate change, pollutants, sedimentation, and other factors, Barott and colleagues hope to continue investigating how such challenges may influence coral reproduction and persistence. Image: Courtesy of Kelsey Speer.

See the full article here .

five-ways-keep-your-child-safe-school-shootings

Please help promote STEM in your local schools.

Stem Education Coalition

U Penn campus

Academic life at University of Pennsylvania (US) is unparalleled, with 100 countries and every U.S. state represented in one of the Ivy League’s most diverse student bodies. Consistently ranked among the top 10 universities in the country, Penn enrolls 10,000 undergraduate students and welcomes an additional 10,000 students to our world-renowned graduate and professional schools.

Penn’s award-winning educators and scholars encourage students to pursue inquiry and discovery, follow their passions, and address the world’s most challenging problems through an interdisciplinary approach.

The University of Pennsylvania (US) is a private Ivy League research university in Philadelphia, Pennsylvania. The university claims a founding date of 1740 and is one of the nine colonial colleges chartered prior to the U.S. Declaration of Independence. Benjamin Franklin, Penn’s founder and first president, advocated an educational program that trained leaders in commerce, government, and public service, similar to a modern liberal arts curriculum.

Penn has four undergraduate schools as well as twelve graduate and professional schools. Schools enrolling undergraduates include the College of Arts and Sciences; the School of Engineering and Applied Science; the Wharton School; and the School of Nursing. Penn’s “One University Policy” allows students to enroll in classes in any of Penn’s twelve schools. Among its highly ranked graduate and professional schools are a law school whose first professor wrote the first draft of the United States Constitution, the first school of medicine in North America (Perelman School of Medicine, 1765), and the first collegiate business school (Wharton School, 1881).

Penn is also home to the first “student union” building and organization (Houston Hall, 1896), the first Catholic student club in North America (Newman Center, 1893), the first double-decker college football stadium (Franklin Field, 1924 when second deck was constructed), and Morris Arboretum, the official arboretum of the Commonwealth of Pennsylvania. The first general-purpose electronic computer (ENIAC) was developed at Penn and formally dedicated in 1946. In 2019, the university had an endowment of $14.65 billion, the sixth-largest endowment of all universities in the United States, as well as a research budget of $1.02 billion. The university’s athletics program, the Quakers, fields varsity teams in 33 sports as a member of the NCAA Division I Ivy League conference.

As of 2018, distinguished alumni and/or Trustees include three U.S. Supreme Court justices; 32 U.S. senators; 46 U.S. governors; 163 members of the U.S. House of Representatives; eight signers of the Declaration of Independence and seven signers of the U.S. Constitution (four of whom signed both representing two-thirds of the six people who signed both); 24 members of the Continental Congress; 14 foreign heads of state and two presidents of the United States, including Donald Trump. As of October 2019, 36 Nobel laureates; 80 members of the American Academy of Arts and Sciences(US); 64 billionaires; 29 Rhodes Scholars; 15 Marshall Scholars and 16 Pulitzer Prize winners have been affiliated with the university.

History

The University of Pennsylvania considers itself the fourth-oldest institution of higher education in the United States, though this is contested by Princeton University(US) and Columbia(US) Universities. The university also considers itself as the first university in the United States with both undergraduate and graduate studies.

In 1740, a group of Philadelphians joined together to erect a great preaching hall for the traveling evangelist George Whitefield, who toured the American colonies delivering open-air sermons. The building was designed and built by Edmund Woolley and was the largest building in the city at the time, drawing thousands of people the first time it was preached in. It was initially planned to serve as a charity school as well, but a lack of funds forced plans for the chapel and school to be suspended. According to Franklin’s autobiography, it was in 1743 when he first had the idea to establish an academy, “thinking the Rev. Richard Peters a fit person to superintend such an institution”. However, Peters declined a casual inquiry from Franklin and nothing further was done for another six years. In the fall of 1749, now more eager to create a school to educate future generations, Benjamin Franklin circulated a pamphlet titled Proposals Relating to the Education of Youth in Pensilvania, his vision for what he called a “Public Academy of Philadelphia”. Unlike the other colonial colleges that existed in 1749—Harvard University(US), William & Mary(US), Yale Unversity(US), and The College of New Jersey(US)—Franklin’s new school would not focus merely on education for the clergy. He advocated an innovative concept of higher education, one which would teach both the ornamental knowledge of the arts and the practical skills necessary for making a living and doing public service. The proposed program of study could have become the nation’s first modern liberal arts curriculum, although it was never implemented because Anglican priest William Smith (1727-1803), who became the first provost, and other trustees strongly preferred the traditional curriculum.

Franklin assembled a board of trustees from among the leading citizens of Philadelphia, the first such non-sectarian board in America. At the first meeting of the 24 members of the board of trustees on November 13, 1749, the issue of where to locate the school was a prime concern. Although a lot across Sixth Street from the old Pennsylvania State House (later renamed and famously known since 1776 as “Independence Hall”), was offered without cost by James Logan, its owner, the trustees realized that the building erected in 1740, which was still vacant, would be an even better site. The original sponsors of the dormant building still owed considerable construction debts and asked Franklin’s group to assume their debts and, accordingly, their inactive trusts. On February 1, 1750, the new board took over the building and trusts of the old board. On August 13, 1751, the “Academy of Philadelphia”, using the great hall at 4th and Arch Streets, took in its first secondary students. A charity school also was chartered on July 13, 1753 by the intentions of the original “New Building” donors, although it lasted only a few years. On June 16, 1755, the “College of Philadelphia” was chartered, paving the way for the addition of undergraduate instruction. All three schools shared the same board of trustees and were considered to be part of the same institution. The first commencement exercises were held on May 17, 1757.

The institution of higher learning was known as the College of Philadelphia from 1755 to 1779. In 1779, not trusting then-provost the Reverend William Smith’s “Loyalist” tendencies, the revolutionary State Legislature created a University of the State of Pennsylvania. The result was a schism, with Smith continuing to operate an attenuated version of the College of Philadelphia. In 1791, the legislature issued a new charter, merging the two institutions into a new University of Pennsylvania with twelve men from each institution on the new board of trustees.

Penn has three claims to being the first university in the United States, according to university archives director Mark Frazier Lloyd: the 1765 founding of the first medical school in America made Penn the first institution to offer both “undergraduate” and professional education; the 1779 charter made it the first American institution of higher learning to take the name of “University”; and existing colleges were established as seminaries (although, as detailed earlier, Penn adopted a traditional seminary curriculum as well).

After being located in downtown Philadelphia for more than a century, the campus was moved across the Schuylkill River to property purchased from the Blockley Almshouse in West Philadelphia in 1872, where it has since remained in an area now known as University City. Although Penn began operating as an academy or secondary school in 1751 and obtained its collegiate charter in 1755, it initially designated 1750 as its founding date; this is the year that appears on the first iteration of the university seal. Sometime later in its early history, Penn began to consider 1749 as its founding date and this year was referenced for over a century, including at the centennial celebration in 1849. In 1899, the board of trustees voted to adjust the founding date earlier again, this time to 1740, the date of “the creation of the earliest of the many educational trusts the University has taken upon itself”. The board of trustees voted in response to a three-year campaign by Penn’s General Alumni Society to retroactively revise the university’s founding date to appear older than Princeton University, which had been chartered in 1746.

Research, innovations and discoveries

Penn is classified as an “R1” doctoral university: “Highest research activity.” Its economic impact on the Commonwealth of Pennsylvania for 2015 amounted to $14.3 billion. Penn’s research expenditures in the 2018 fiscal year were $1.442 billion, the fourth largest in the U.S. In fiscal year 2019 Penn received $582.3 million in funding from the National Institutes of Health(US).

In line with its well-known interdisciplinary tradition, Penn’s research centers often span two or more disciplines. In the 2010–2011 academic year alone, five interdisciplinary research centers were created or substantially expanded; these include the Center for Health-care Financing; the Center for Global Women’s Health at the Nursing School; the $13 million Morris Arboretum’s Horticulture Center; the $15 million Jay H. Baker Retailing Center at Wharton; and the $13 million Translational Research Center at Penn Medicine. With these additions, Penn now counts 165 research centers hosting a research community of over 4,300 faculty and over 1,100 postdoctoral fellows, 5,500 academic support staff and graduate student trainees. To further assist the advancement of interdisciplinary research President Amy Gutmann established the “Penn Integrates Knowledge” title awarded to selected Penn professors “whose research and teaching exemplify the integration of knowledge”. These professors hold endowed professorships and joint appointments between Penn’s schools.

Penn is also among the most prolific producers of doctoral students. With 487 PhDs awarded in 2009, Penn ranks third in the Ivy League, only behind Columbia University(US) and Cornell University(US) (Harvard University(US) did not report data). It also has one of the highest numbers of post-doctoral appointees (933 in number for 2004–2007), ranking third in the Ivy League (behind Harvard and Yale University(US)) and tenth nationally.

In most disciplines Penn professors’ productivity is among the highest in the nation and first in the fields of epidemiology, business, communication studies, comparative literature, languages, information science, criminal justice and criminology, social sciences and sociology. According to the National Research Council nearly three-quarters of Penn’s 41 assessed programs were placed in ranges including the top 10 rankings in their fields, with more than half of these in ranges including the top five rankings in these fields.

Penn’s research tradition has historically been complemented by innovations that shaped higher education. In addition to establishing the first medical school; the first university teaching hospital; the first business school; and the first student union Penn was also the cradle of other significant developments. In 1852, Penn Law was the first law school in the nation to publish a law journal still in existence (then called The American Law Register, now the Penn Law Review, one of the most cited law journals in the world). Under the deanship of William Draper Lewis, the law school was also one of the first schools to emphasize legal teaching by full-time professors instead of practitioners, a system that is still followed today. The Wharton School was home to several pioneering developments in business education. It established the first research center in a business school in 1921 and the first center for entrepreneurship center in 1973 and it regularly introduced novel curricula for which BusinessWeek wrote, “Wharton is on the crest of a wave of reinvention and change in management education”.

Several major scientific discoveries have also taken place at Penn. The university is probably best known as the place where the first general-purpose electronic computer (ENIAC) was born in 1946 at the Moore School of Electrical Engineering.

ENIAC UPenn

It was here also where the world’s first spelling and grammar checkers were created, as well as the popular COBOL programming language. Penn can also boast some of the most important discoveries in the field of medicine. The dialysis machine used as an artificial replacement for lost kidney function was conceived and devised out of a pressure cooker by William Inouye while he was still a student at Penn Med; the Rubella and Hepatitis B vaccines were developed at Penn; the discovery of cancer’s link with genes; cognitive therapy; Retin-A (the cream used to treat acne), Resistin; the Philadelphia gene (linked to chronic myelogenous leukemia) and the technology behind PET Scans were all discovered by Penn Med researchers. More recent gene research has led to the discovery of the genes for fragile X syndrome, the most common form of inherited mental retardation; spinal and bulbar muscular atrophy, a disorder marked by progressive muscle wasting; and Charcot–Marie–Tooth disease, a progressive neurodegenerative disease that affects the hands, feet and limbs.

Conductive polymer was also developed at Penn by Alan J. Heeger, Alan MacDiarmid and Hideki Shirakawa, an invention that earned them the Nobel Prize in Chemistry. On faculty since 1965, Ralph L. Brinster developed the scientific basis for in vitro fertilization and the transgenic mouse at Penn and was awarded the National Medal of Science in 2010. The theory of superconductivity was also partly developed at Penn, by then-faculty member John Robert Schrieffer (along with John Bardeen and Leon Cooper). The university has also contributed major advancements in the fields of economics and management. Among the many discoveries are conjoint analysis, widely used as a predictive tool especially in market research; Simon Kuznets’s method of measuring Gross National Product; the Penn effect (the observation that consumer price levels in richer countries are systematically higher than in poorer ones) and the “Wharton Model” developed by Nobel-laureate Lawrence Klein to measure and forecast economic activity. The idea behind Health Maintenance Organizations also belonged to Penn professor Robert Eilers, who put it into practice during then-President Nixon’s health reform in the 1970s.

International partnerships

Students can study abroad for a semester or a year at partner institutions such as the London School of Economics(UK), University of Barcelona [Universitat de Barcelona](ES), Paris Institute of Political Studies [Institut d’études politiques de Paris](FR), University of Queensland(AU), University College London(UK), King’s College London(UK), Hebrew University of Jerusalem(IL) and University of Warwick(UK).